Microarray:Article Title: Biodegradable Meshes Printed with Extracellular Matrix Proteins Support Micropatterned Hepatocyte Cultures
Article Snippet: .. Position of the pure Fn spot is indicated, and the box shows its nearest extracellular matrix (ECM) neighbors. ( B ) Diagram showing pattern of protein deposition for each of the six ECM proteins used to print the microarray. ( C ) Proteins were deposited in four replicates onto HydroGel microarray slides using a Virtek ChipWriter Pro robotic microarrayer. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 ID Collagen I (C1) Collagen III (C3) Collagen IV (C4) Fibronectin (Fn) Laminin (Ln) EHS Matrix (E) No. of ECM proteins 1 Y N N N N N 1 2 N Y N N N N 3 N N Y N N N 4 N N N Y N N 5 N N N N Y N 6 N N N N N Y 7 Y Y N N N N 2 8 Y N Y N N N 9 Y N N Y N N 10 Y N N N Y N 11 Y N N N N Y 12 N Y Y N N N 13 N Y N Y N N 14 N Y N N Y N 15 N Y N N N Y 16 N N Y Y N N 17 N N Y N Y N 18 N N Y N N Y 19 N N N Y Y N 20 N N N Y N Y 21 N N N N Y Y 22 Y Y Y N N N 3 23 Y Y N Y N N 24 Y Y N N Y N 25 Y Y N N N Y 26 Y N Y Y N N 27 Y N Y N Y N 28 Y N Y N N Y 29 Y N N Y Y N 30 Y N N Y N Y 31 Y N N N Y Y 32 N Y Y Y N N 33 N Y Y N Y N 34 N Y Y N N Y 35 N Y N Y Y N 36 N Y N Y N Y 37 N Y N N Y Y 38 N N Y Y Y N 39 N N Y Y N Y 40 N N Y N Y Y 41 N N N Y Y Y 42 N N Y Y Y Y 4 43 N Y N Y Y Y 44 N Y Y N Y Y 45 N Y Y Y N Y 46 N Y Y Y Y N 47 Y N N Y Y Y 48 Y N Y N Y Y 49 Y N Y Y N Y 50 Y N Y Y Y N 51 Y Y N N Y Y 52 Y Y N Y N Y 53 Y Y N Y Y N 54 Y Y Y N N Y 55 Y Y Y N Y N 56 Y Y Y Y N N 57 N Y Y Y Y Y 5 58 Y N Y Y Y Y 59 Y Y N Y Y Y 60 Y Y Y N Y Y 61 Y Y Y Y N Y 62 Y Y Y Y Y N 63 N N N N N N 0 64 Y Y Y Y Y Y 6 Open in a separate window Components of Extracellular Matrix (ECM) Combinatorial Array Indirect immunofluorescence Protein immobilization on the microarray substrate was evaluated using indirect immunofluorescence. .. Position of the pure Fn spot is indicated, and the box shows its nearest extracellular matrix (ECM) neighbors. ( B ) Diagram showing pattern of protein deposition for each of the six ECM proteins used to print the microarray. ( C ) Proteins were deposited in four replicates onto HydroGel microarray slides using a Virtek ChipWriter Pro robotic microarrayer.
Article Title: Endothelial cell micropatterning: Methods, effects, and applications
Article Snippet: 60 Using a modified Hewlett-Packard inkjet printer, Gauvreau et al. were able to print CRGD, GRGDS and WQPPRARI peptides as well as their combinations onto plasma-treated expanded polytetrafluoroethylene (ePTFE). .. 26 Similar results were obtain by Woodrow et al. using a modified robotic microarray printer (Virtek ChipWriter Pro, BioRad, Hercules, CA) to print proteins singularly and in combination, specifically collagen I, collagen III, collagen IV, FN, laminin, and Matrigel (a solution of basement membrane proteins), onto electrospun PLA scaffolds or onto a HydroGel slide (a commercially available acrylamide surface on glass). ..
Immunofluorescence:Article Title: Biodegradable Meshes Printed with Extracellular Matrix Proteins Support Micropatterned Hepatocyte Cultures
Article Snippet: .. Position of the pure Fn spot is indicated, and the box shows its nearest extracellular matrix (ECM) neighbors. ( B ) Diagram showing pattern of protein deposition for each of the six ECM proteins used to print the microarray. ( C ) Proteins were deposited in four replicates onto HydroGel microarray slides using a Virtek ChipWriter Pro robotic microarrayer. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 ID Collagen I (C1) Collagen III (C3) Collagen IV (C4) Fibronectin (Fn) Laminin (Ln) EHS Matrix (E) No. of ECM proteins 1 Y N N N N N 1 2 N Y N N N N 3 N N Y N N N 4 N N N Y N N 5 N N N N Y N 6 N N N N N Y 7 Y Y N N N N 2 8 Y N Y N N N 9 Y N N Y N N 10 Y N N N Y N 11 Y N N N N Y 12 N Y Y N N N 13 N Y N Y N N 14 N Y N N Y N 15 N Y N N N Y 16 N N Y Y N N 17 N N Y N Y N 18 N N Y N N Y 19 N N N Y Y N 20 N N N Y N Y 21 N N N N Y Y 22 Y Y Y N N N 3 23 Y Y N Y N N 24 Y Y N N Y N 25 Y Y N N N Y 26 Y N Y Y N N 27 Y N Y N Y N 28 Y N Y N N Y 29 Y N N Y Y N 30 Y N N Y N Y 31 Y N N N Y Y 32 N Y Y Y N N 33 N Y Y N Y N 34 N Y Y N N Y 35 N Y N Y Y N 36 N Y N Y N Y 37 N Y N N Y Y 38 N N Y Y Y N 39 N N Y Y N Y 40 N N Y N Y Y 41 N N N Y Y Y 42 N N Y Y Y Y 4 43 N Y N Y Y Y 44 N Y Y N Y Y 45 N Y Y Y N Y 46 N Y Y Y Y N 47 Y N N Y Y Y 48 Y N Y N Y Y 49 Y N Y Y N Y 50 Y N Y Y Y N 51 Y Y N N Y Y 52 Y Y N Y N Y 53 Y Y N Y Y N 54 Y Y Y N N Y 55 Y Y Y N Y N 56 Y Y Y Y N N 57 N Y Y Y Y Y 5 58 Y N Y Y Y Y 59 Y Y N Y Y Y 60 Y Y Y N Y Y 61 Y Y Y Y N Y 62 Y Y Y Y Y N 63 N N N N N N 0 64 Y Y Y Y Y Y 6 Open in a separate window Components of Extracellular Matrix (ECM) Combinatorial Array Indirect immunofluorescence Protein immobilization on the microarray substrate was evaluated using indirect immunofluorescence. .. Position of the pure Fn spot is indicated, and the box shows its nearest extracellular matrix (ECM) neighbors. ( B ) Diagram showing pattern of protein deposition for each of the six ECM proteins used to print the microarray. ( C ) Proteins were deposited in four replicates onto HydroGel microarray slides using a Virtek ChipWriter Pro robotic microarrayer.
Modification:Article Title: Endothelial cell micropatterning: Methods, effects, and applications
Article Snippet: 60 Using a modified Hewlett-Packard inkjet printer, Gauvreau et al. were able to print CRGD, GRGDS and WQPPRARI peptides as well as their combinations onto plasma-treated expanded polytetrafluoroethylene (ePTFE). .. 26 Similar results were obtain by Woodrow et al. using a modified robotic microarray printer (Virtek ChipWriter Pro, BioRad, Hercules, CA) to print proteins singularly and in combination, specifically collagen I, collagen III, collagen IV, FN, laminin, and Matrigel (a solution of basement membrane proteins), onto electrospun PLA scaffolds or onto a HydroGel slide (a commercially available acrylamide surface on glass). ..
Membrane:Article Title: Endothelial cell micropatterning: Methods, effects, and applications
Article Snippet: 60 Using a modified Hewlett-Packard inkjet printer, Gauvreau et al. were able to print CRGD, GRGDS and WQPPRARI peptides as well as their combinations onto plasma-treated expanded polytetrafluoroethylene (ePTFE). .. 26 Similar results were obtain by Woodrow et al. using a modified robotic microarray printer (Virtek ChipWriter Pro, BioRad, Hercules, CA) to print proteins singularly and in combination, specifically collagen I, collagen III, collagen IV, FN, laminin, and Matrigel (a solution of basement membrane proteins), onto electrospun PLA scaffolds or onto a HydroGel slide (a commercially available acrylamide surface on glass). ..
Proximity Ligation Assay:Article Title: Endothelial cell micropatterning: Methods, effects, and applications
Article Snippet: 60 Using a modified Hewlett-Packard inkjet printer, Gauvreau et al. were able to print CRGD, GRGDS and WQPPRARI peptides as well as their combinations onto plasma-treated expanded polytetrafluoroethylene (ePTFE). .. 26 Similar results were obtain by Woodrow et al. using a modified robotic microarray printer (Virtek ChipWriter Pro, BioRad, Hercules, CA) to print proteins singularly and in combination, specifically collagen I, collagen III, collagen IV, FN, laminin, and Matrigel (a solution of basement membrane proteins), onto electrospun PLA scaffolds or onto a HydroGel slide (a commercially available acrylamide surface on glass). ..
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